Bio 679 : Protein Chemistry and Enzymology
Taught by Pr. Manickam Sugumaran
Wednesdays 4:30-7:30pm, M-1-318
| Important
Dates |
Lecture
Topics |
Slides |
Important
Information |
| Jan. 27 |
Introductory class. | |
|
| Feb. 1 | Add/Drop ends |
||
| Feb. 3 |
Separation technology; Separation and purification of proteins based on their size, charge, biological affinity and other physicochemical properties. | |
|
| Feb. 10 | Immobilization of enzymes and use of immobilized enzymes. | |
|
| Feb 15 | |
President’s day
- Holiday |
|
| Feb. 17 |
Primary structure of proteins, amino acid analysis; protein sequencing; peptide synthesis. | |
|
| Feb. 24 |
Secondary, tertiary and quaternary structure of proteins. | |
|
| Mar. 3 |
Enzyme classification; enzyme kinetics – single substrate rxns; inhibition studies; multi-substrate rxns. | (Take home exam due)
|
|
| Mar. 10 |
pH and temperature effects on enzymes; thermo-stability of enzymes and enzymes in non-aqueous solution. | |
|
| Mar. 13-21 | |
Spring Break |
|
| Mar. 17 |
Evacuation day. | |
|
| Mar. 24 | Regulation of enzyme activity; allosteric
models |
(Take home exam due)
|
|
| Mar. 31 | Active site labeling; chemical modification
of proteins; suicidal inactivation. |
|
|
| Apr. 1 | Pass/Fail and Course
withdrawal deadline. |
||
| Apr. 7 | Post-translational modification of proteins. | |
|
| Apr.14 | Catalytic antibodies; Combinatorial chemistry | |
|
| Apr. 19 |
|
Patriot’s day
- Holiday |
|
| Apr. 21 |
Ribozymes. coenzymes | |
|
| Apr. 28 |
Enzyme mechanisms | |
|
| May 5 |
(Take home exam due) Drug discovery through protein chemistry; Proteomics. | |
|
| May 12 | Classes end. Student presentations. | |
|
| May 13-16 |
Study Period | |
|
| May 17-21 |
Final Exams (No exam) Final Paper due. | |
|
| May 25 | Grades Due |
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